'He has had a calf injury all week and he has done well to play tonight,' Ferguson told Sky.
'But we have had to withdraw him from the international team.
'He has got a little pull in his left calf muscle which obviously is a concern for us because we have Bolton next Wednesday.'
Earlier today, Blackburn's David Dunn was called up to the full England squad as a replacement for Newcastle winger Kieron Dyer.
The Football Association revealed coach Sven-Goran Eriksson would not decide until Wednesday whether to call up a replacement for Beckham.