It is hoped the damage is not too serious, but is enough to keep two of Ferguson's most important players out of a key domestic encounter.
"Unfortunately, Nani has a groin injury and is out for Saturday," confirmed Ferguson.
"I think Darren will be out as well, so that part has been disappointing.
"It is disappointing but these things happen in football. We may have Darron Gibson back after a bout of flu but Anderson has had a virus as well and I don't think he will be ready."
Despite his disappointment, Ferguson insists he will be happy to Gabriel Obertan and Bebe against Wolves, both players found the net last night.
Obertan was scoring his first United goal in only his second start of the campaign, whilst Bebe followed up his effort against Wolves in the Carling Cup last week by tucking home Paul Scholes' clever pass.
"Gabriel has been derailed quite a bit by injury," Ferguson said.
"It has been a problem for the lad. He had two or three last season and then picked up another in Texas this pre-season that kept him out for five or six weeks. But he is back now and as he showed tonight, he has fantastic talent."